Site-selective depurination by a periodate-dependent deoxyribozyme

Site-selective depurination by a periodate-dependent deoxyribozyme.

A deoxyribozyme is identified that mediates the site-selective depurination of its 5'-terminal guanosine nucleotide using periodate (IO(4)-) as an obligatory cofactor.

Engineering a selective small-molecule substrate binding site into a deoxyribozyme.

Selective radioactive labeling of cell surface sialoglycoproteins by periodate-tritiated borohydride.

Low concentrations of sodium metaperiodate induce specific oxidative cleavage of sialic acids between carbon 7 and carbon 8 or carbon 8 and carbon 9. The aldehydes formed can easily be reduced with NaB3H4 to tritiated 5-acetamido-3,5-dideoxy-L-arabino-2-heptulosonic acid or 5-acetamido-3,5-dideoxy-L-arabino-2-octulosonic acid. At 0 degrees, the periodate anion penetrates the cell plasma membran...

Depurination of N7-methylguanine by DNA glycosylase AlkD is dependent on the DNA backbone.

DNA glycosylase AlkD excises N7-methylguanine (7mG) by a unique but unknown mechanism, in which the damaged nucleotide is positioned away from the protein and the phosphate backbone is distorted. Here, we show by methylphosphonate substitution that a phosphate proximal to the lesion has a significant effect on the rate enhancement of 7mG depurination by the enzyme. Thus, instead of a convention...

Quantitative Determination of Dihydrostreptomycin by Periodate Oxidation.

Dihydrostreptomycin is produced commercially by the catalytic hydrogenation of streptomycin. The reaction involves the reduction of the aldehyde group in the streptose moiety of the streptomycin molecule to the corresponding primary carbinol group as evidenced by the failure of dihydrostreptomycin to react with carbonyl reagents or with alkali to produce maltol. As there are no chemical methods...